张炳坤

张炳坤，博士，博士生导师，中国农业大学动物科技学院教授,农业农村部饲料安全与生物学效价重点实验室副主任，国家重点研发计划政府间重点专项首席科学家。中国畜牧兽医学会动物营养分会理事、北京畜牧兽医学会理事、World's Poultry Science Association (WPSA)、美国American Gastroenterological Association (AGA)会员等。曾在美国芝加哥大学（The University of Chicago）进行病原菌调控宿主肠道屏障功能作用机制合作研究。

聚焦肠黏膜屏障功能健康发育的营养调控及其相关机制研究。长期致力于促进动物黏膜屏障功能健康发育的营养调控及其相关机制研究。近年来，揭示了紧密连接蛋白claudin-2在抵抗细菌感染的具体作用机制。发现病原菌通过改变紧密连接蛋白表达，破坏肠道屏障功能；细菌感染通过细胞因子IL-22介导肠道紧密连接蛋白claudin-2表达的提高、增加紧密连接通透性、促进Na+离子和水的排泄，最终实现清除病菌；首次建立了体内评价肠道通透性大小的多探针检测技术。建立了单层细胞肠上皮和organoid培养体系，揭示了锌具有抵抗疾病感染、维持肠上皮组织完整性等重要作用的信号传导机制、丰富了锌营养理论。发现精氨酸需要通过促进肠道基质细胞分泌Wnt2b、激活Wnt/β-catenin信号通路，才能提高肠道Lgr5+细胞更新功能、降低肠道炎症损伤。利用实时无标记细胞分析仪（RTCA）构建了植物提取物活性功能体外筛选模型，并筛选出具有抗氧化活性的天然有效成分。证实了这些活性单体通过激活Nrf2，增加SOD、CAT、GCS转录、提高Gpx活力、减少ROS产生，从而改善细胞受损，提高细胞活率，实现缓解肉鸡肠道氧化应激作用。

目前主持国家自然科学基金、国家重点研发计划课题、国际合作项目等。

获教育部科学技术进步一等奖和国家科学技术进步二等奖各1项，省部级教学成果二等奖1项，起草制定《产蛋后备鸡、产蛋鸡、肉用仔鸡配合饲料》、《玉米干全酒糟（玉米DDGS）》、蛋鸡、肉鸡配合饲料等国家或行业标准以及《饲料添加剂安全使用规范》，参编《家禽营养》、《动物免疫营养学》等著作，近期已在国际著名期刊Cell Host & Microbe 、Journal of Crohn’s and Colitis、Pharmacological Research、SCIENCE CHINA Life Sciences、FASEB Journal、Frontiers in Immunology、Communications Biology、Journal of Nutrition 、Journal of Nutritional Biochemistry、Journal of Agricultural and Food Chemistry、British Journal of Nutrition、Animal Feed Science & Technology、Poultry Science、Animal、Avian Pathology等期刊上发表论文，申请授权发明专利6项，获得授权发明专利8项。

  研究方向

肠道屏障功能和黏膜免疫在肠炎中的作用；

肠道健康的营养调控（植物提取物）；

畜禽产品品质的调节；

新型饲料添加剂开发与应用。

代表研究内容

IL-22 upregulates epithelial claudin-2 to drive diarrhea and enteric pathogen clearance

Regulation of the Paneth cell niche by exogenous L-arginine couples the intestinal stem cell function

    Exogenous L-arginine increases intestinal stem cell function through CD90+ stromal cells producing mTORC1-induced Wnt2b

    Intestinal stem cells and immune cells relationships: potential therapeutic targets for inflammatory bowel diseases

    Quercetin alleviates intestinal oxidative damage induced by H2O2 via modulation of GSH: In vitro screening and in vivo evaluation in a colitis model

代表论文

Jiang Qiuyu, Lihua Zhao, Jiaqi Lei, Xiangfei Geng, Xiang Zhong, Bingkun Zhang*. Interaction between energy level and starch: fat ratio on intestinal energy metabolism of layer pullets. Animal Nutrition. 2024.

Hou Qihang, Guang Li, Xianjie Pan, Xiang Zhong, Xiangfei Geng, Xin Yang, Xiaojun Yang, Bingkun Zhang*. Long-term supplementation of genistein improves immune homeostasis in the aged gut and extends the laying cycle of aged laying hens. Poultry Science. 2024. 103(6): 103670. 

Liao Chaoyong, Jian Cui, Mingkun Gao, Bo Wang, Koichi Ito, Yuming Guo, Bingkun Zhang*. Dual-sgRNA CRISPRa system for enhanced MK-7 production and Salmonella infection mitigation in Bacillus subtilis natto applied to Caco-2 cells. Journal of Agricultural and Food Chemistry. 2024. 72(8): 4301-4316. 

Wei Yi, Yongfa Liu, Guang Li, Yuming Guo, Bingkun Zhang*. Effects of quercetin and genistein on egg quality, lipid profiles, and immunity in laying hens. Journal of the Science of Food and Agriculture. 2024. 104: 207-214.

Li Guang#, Yuqing Feng#, Jian Cui, Qihang Hou, Tanfang Li, Meiting Jia, Zhengtian Lv, Qiuyu Jiang, Ying Wang, Ming Zhang, Lin Wang, Zengpeng Lv, Junyou Li, Yuming Guo*, Bingkun Zhang*. The ionome and proteome landscape of aging in laying hens and relation to egg white quality. SCIENCE CHINA Life Sciences. 2023. 66 (9) : 2020-2040.

Li Guang, Siyuan Mi, Qi Zeng, Lin Wang, Xingbo Liu, Ming Zhang, Zengpeng Lv, Yongguo Jin, Junyou Li, Yuming Guo, Bingkun Zhang*. Quantitative proteomics provides insights into the mechanism of the differences in heat-induced gel properties for egg white proteins with different interior quality during ageing in laying hens. Food Chemistry. 2023. 419(1): 136031. 

i Guang, Xinyue Yang, Junyou Li, Bingkun Zhang*. Genome-wide analysis of lncRNA and mRNA expression in the uterus of laying hens during aging. Genes. 2023. 14(3): 639.

Hou Qihang#, Jingxi Huang#, Lihua Zhao, Xianjie Pan, Chaoyong Liao, Qiuyu Jiang, Jiaqi Lei, Fangshen Guo, Jian Cui, Yuming Guo, Bingkun Zhang*. Dietary genistein increases microbiota-derived short chain fatty acid levels, modulates homeostasis of the aging gut, and extends healthspan and lifespan. Pharmacological Research. 2023. 188: 106676.

Ayansola Hammed, Yanhui Luo, Yan Wan, Xiaoxiao Yu, Jiaqi Lei, Kewei Yu, Chaoyong Liao, Yuming Guo, Bingkun Zhang*, Bo Wang*. Restricted feeding regimens improve white striping associated muscular defects in broiler chickens. Animal Nutrition. 2023. 12: 128-137. 

Wan Yan, Bingkun Zhang*. The impact of zinc and zinc homeostasis on the intestinal mucosal barrier and intestinal diseases. Biomolecules. 2022. 12(7): 900. 

Jiang Qiuyu, Wei Wu, Yan Wan, Yi Wei, Yoichiro Kawamura, Junyou Li, Yuming Guo, Zhibin Ban, Bingkun Zhang*. Energy values evaluation and improvement of soybean meal in broiler chickens through supplemental mutienzyme. Poultry Science. 2022. 101(8): 101978. 

Lei Jiaqi, Yuanyang Dong, Qihang Hou, Yang He, Yujiao Lai, Chaoyong Liao, Yoichiro Kawamura, Junyou Li, Bingkun Zhang*. Intestinal microbiota regulate certain meat quality parameters in chicken. Frontiers in Nutrition. 2022. 9: 747705. 

Hou Qihang, Jingxi Huang, Xia Xiong, Yuming Guo, Bingkun Zhang*. Role of nutrient-sensing receptor GPRC6A in regulating colonic group 3 innate lymphoid cells and inflamed mucosal healing. Journal of Crohn’s and Colitis. 2022. 16(8): 1293-1305.

Lai Yujiao, Meng Sun, Yang He, Jiaqi Lei, Yanming Han, Yuanyuan Wu, Dongying Bai, Yuming Guo, Bingkun Zhang*. Mycotoxins binder supplementation alleviates aflatoxin B1 toxic effects on the immune response and intestinal barrier function in broilers. Poultry Science. 2022. 101(3): 101683. 

Lai Yujiao, Hori Masatoshi, Yanbo Ma, Yuming Guo, Bingkun Zhang*. Role of vitamin K in intestinal health. Frontiers in Immunology. 2022. 12:791565. 

Jiang Qiuyu, Jingjing Sun, Yang He, Yanbo Ma, Bingkun Zhang*, Yanming Han, Yuanyuan Wu. Hydroxychloride trace elements improved eggshell quality partly by modulating uterus histological structure and inflammatory cytokines expression in aged laying hens. Poultry Science. 2021. 100(11): 101453. 

Liao Chaoyong, Ayansola Hammed, Yanbo Ma, Koichi Ito, Yuming Guo, Bingkun Zhang*. Advances in enhanced menaquinone-7 production from Bacillus subtilis. Frontiers in Bioengineering and Biotechnology. 2021. 9: 695526.

He Yang #, Xuan Liu#, Yuanyang Dong, Jiaqi Lei, Koichi Ito, Bingkun Zhang*. Enterococcus faecium PNC01 isolated from the intestinal mucosa of chicken as an alternative for antibiotics to reduce feed conversion rate in broiler chickens. Microbial Cell Factories. 2021. 20: 122. 

He Yang, Hammed Ayansola, Yujiao Lai, Qihang Hou, Chaoyong Liao, Jiaqi Lei, Qiuyu Jiang, Hori Masatoshi, Bingkun Zhang*. Genistein inhibits colonic goblet cell loss and colorectal inflammation induced by Salmonella Typhimurium infection. Molecular Nutrition & Food Research. 2021. 65(16): 202100209.

Qihang Hou, Jingxi Huang, Hammed Ayansola, Hori Masatoshi, Bingkun Zhang*. Intestinal stem cells and  immune cells relationships: potential therapeutic targets for inflammatory bowel diseases. Frontiers in Immunology. 11:623691. doi: 10.3389/fimmu.2020.623691

He Yang#, Yanyan Yang#, Yuanyang Dong, Koichi Ito, Bingkun Zhang*. Highly nutritious diet resists  Salmonella Typhimurium infections by improving intestinal microbiota and morphology in broiler chickens. Poultry Science. 2020. 99(12): 7055-7065. December 2020. https://doi.org/10.1016/j.psj.2020.09.073

Qihang Hou, Yuanyang Dong, Jingxi Huang, Chaoyong Liao, Jiaqi Lei, Youli Wang, Yujiao Lai, Yifei Bian, Yang He, Jingjing Sun, Meng Sun, Qiuyu Jiang, Bo Wang, Zhengquan Yu, Yuming Guo, Bingkun Zhang*. Exogenous L-arginine increases intestinal stem cell function through CD90+ stromal cells producing mTORC1-induced Wnt2b. Communications Biology. 2020. 3: 611. https://doi.org/10.1038/s42003-020-01347-9

Yuanyang Dong, Jiaqi Lei, Bingkun Zhang*. Dietary Quercetin Alleviated DSS-induced Colitis in Mice through Several Possible Pathways by Transcriptome Analysis. Current Pharmaceutical Biotechnology. 2020. 21:1-9.

Hou Qihang, Yuanyang Dong, Qinghua Yu, Bo Wang, Shen Le, Yuming Guo, Bingkun Zhang*. Regulation of the Paneth cell niche by exogenous L-arginine couples the intestinal stem cell function. The FASEB Journal. 2020;34:10299–10315.

Dong Yuanyang, Qihang Hou, Meng Sun, Jingjing Sun, Bingkun Zhang*. Targeted Isolation of Antioxidant Constituents from Plantago asiatica L. and In Vitro Activity Assay. Molecules. 2020. 25(8), 1825.

Dong Yuanyang, Qihang Hou, Jiaqi Lei, Patricia G. Wolf, Hammed Ayansola, Bingkun Zhang*. Quercetin alleviates intestinal oxidative damage induced by H₂O₂ via modulation of GSH: In vitro screening and in vivo evaluation in a colitis model of mice. ACS Omega. 2020. 5, 14, 8334-8346. 

Bian Yifei, Yuanyang Dong, Jingjing Sun, Meng Sun, Qihang Hou, Yujiao Lai, Bingkun Zhang*. Protective effect of kaempferol on LPS-induced inflammation and barrier dysfunction in a coculture model of intestinal epithelial cells and intestinal microvascular endothelial cells. Journal of Agricultural and Food Chemistry. 2020. 68(1): 160-167.

He Yang, Yanyan Yang, Yuanyang Dong, Changling Yan, Bingkun Zhang*. The Effects of flavomycin and colistin sulfate pre-treatment on ileal bacterial community composition, the response to Salmonella typhimurium and host gene expression in broiler chickens. Microorganisms 2019. 7(11): 574.

Tan Lulin, Deng Rong, Yanyan Yang, Bingkun Zhang*.The effect of oxidized fish oils on growth performance, oxidative status and intestinal barrier function in broiler chickens.2019. Journal of Applied Poultry Research. 28(1): 31-41.

Wei Wu, Zhibin Xiao, Wenyi An, Yuanyang Dong, Bingkun Zhang*. Dietary sodium butyrate improves intestinal development and function by modulating the microbial community in broilers. PLoS ONE 2018. 13(5): e0197762.

Tan L, Rong D, Yang Y, Zhang B*. Effect of oxidized soybean oils on oxidative status and intestinal barrier function in broiler chickens. 2018. Brazilian Journal of Poultry Science. 20(2):333-342.

Tsai Pei-Yun#, Bingkun Zhang#, Wei-Qi He, Juan-Min Zha, Matthew A. Odenwald, Gurminder Singh, Atsushi Tamura, Le Shen, Anne Sailer, Sunil Yeruva, Wei-Ting Kuo, Yang-Xin Fu, Sachiko Tsukita, and Jerrold R. Turner. 2017. IL-22 upregulates epithelial claudin-2 to drive diarrhea and enteric pathogen clearance. Cell Host & Microbe. 21(6): 671–681.

Shao Yu-Xin, Zhao Lei, Patricia G Wolf, Yan Gao, Yu-Ming Guo, and Bing-Kun Zhang*. 2017. Zinc Supplementation, via GPR39, Upregulates PKC zeta to Protect Intestinal Barrier Integrity in Caco-2 Cells Challenged by Salmonella enterica Serovar Typhimurium. The Journal of Nutrition. 147(7):1282-1289.

Shao Yuxin, Patricia G. Wolf, Shuangshuang Guo, Yuming Guo, H. Rex Gaskinsc, Bingkun Zhang*. 2017. Zinc enhances intestinal epithelial barrier function through the PI3K/AKT/mTOR signaling pathway in Caco-2 cells. Journal of Nutritional Biochemistry. 43: 18–26.

Shao Y., Z. Lei, J. Yuan,Y. Yang, Y. Guo, and B. Zhang*. 2014. Effect of zinc on growth performance, gut morphometry, and cecal microbial community in broilers challenged with Salmonella enterica serovar Typhimurium. J Microbiol. 52(12): 1002–1011.

Zhang B.#, Y. Shao, D. Liu, P. Yin, Y. Guo, and J. Yuan. 2012. Zinc prevents Salmonella enterica Serovar Typhimurium-induced loss of intestinal mucosal barrier function in broiler chickens. Avian Pathol. 41(4): 361-367.

Zhang Bingkun#, Li Haitao, Dongqin Zhao, Yuming Guo, Adriana Barri. 2011. Effect of fat type and lysophosphatidylcholine addition to broiler diets on performance, apparent digestibility of fatty acids, and apparent metabolizable energy content. Anim. Feed. Sci. Tech. 163: 177-184.

Zhang Bingkun#; X. Yang; Y. Guo; F. Long. 2011. Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens. Animal. 5(12): 1909-1915.

Zhang B. K.#, and Y. M. Guo. 2009. Supplemental zinc reduced intestinal permeability by enhancing occludin and ZO-1 expression in weaning piglets. Brit. J. Nutr.102(5): 687–693.

孙静静, 张炳坤*. 家禽微量元素敏感指标研究进展. 动物营养学报. 2021，33( 4).2020-12-15 15:49

万妍，邵玉新，张炳坤*.不同阶段日粮锌营养对肉鸡生长性能及肠黏膜屏障功能的影响. 中国家禽. 2020.42(9) : 54-61. 

江秋雨，何阳，呙于明，张炳坤*. 家禽净能体系的构建及其在饼粕类饲料上的应用. 动物营养学报. 2020. 32(9) : 4020-4028. 

万妍，许彦飞，董元洋，张炳坤*. 超微粉碎车前对沙门氏菌感染肉仔鸡生长性能、免疫功能和抗氧化功能的影响. 动物营养学报. 2020. 32 (8): 3636-3653.

安文艺，雷佳琦，董元洋，武威，刘璇，邵玉新，张炳坤*. 壳寡糖与屎肠球菌对肉仔鸡生长性能、免疫功能及肠道短链脂肪酸含量的影响. 中国畜牧杂志. 2019. 55(08):110-117. 

荣誉及奖励

  张炳坤，袁建敏.以雏鹰俱乐部作为技术依托的创新创业人才培养模式. 全国农业专业学位研究生实践教学成果奖二等奖. 2018.10.17. 全国农业专业学位研究生教育指导委员会.

肉鸡健康养殖的营养调控与饲料高效利用技术. 呙于明(#); 张日俊; 李绍钰; 吕明斌; 袁建敏; 郝国庆; 杨鹰; 魏凤仙; 张炳坤; 王忠. 国家科学技术奖励工作办公室,国家科技进步二等奖, 2011.12.23.

中国畜牧兽医学会动物营养分会理事

北京畜牧兽医学会理事

American Gastroenterological Association (AGA)

World's Poultry Science Association (WPSA)

肠道屏障功能和黏膜免疫在肠炎中的作用；

肠道健康的营养调控；

畜禽产品品质的调节；

新型饲料添加剂开发与应用。