方美英

方美英，中国农业大学动物科学技术学院教授、博士生导师，教育部“新世纪优秀人才支持计划”入选者，先后赴瑞典农业大学、美国康奈尔大学工作与访学交流。长期深耕畜禽遗传改良、畜禽优异种质特性挖掘与遗传机理解析研究，主导完成了中国五指山小型猪T2T无间隙基因组组装，成果以封面文章发表于《Nature Genetics》，为五指山猪特殊体长性状的遗传改良提供了基础。

现担任全国科技平台标准化技术委员会委员；国家畜禽遗传资源委员会猪专委会委员；中国动物遗传育种学会及全国动物生物技术学会理事；第三次全国猪遗传资源普查技术专家；海南省畜禽遗传资源委员会猪专委会委员；畜禽遗传改良北京市重点实验室副主任等工作。主持多项国家自然科学基金、国家“863”计划、国家科技重大专项以及中乌政府间国际合作等课题；参与完成了国家“973”和欧盟第七框架等科研项目。以第一作者/通讯作者发表学术论文九十余篇，授权国家发明专利十余项。主编《家养动物驯化与品种培育》和《畜禽遗传资源保护与利用》，参编“十二五”普通高等教育本科国家级规划教材《动物遗传学》（第二版）及《动物种业科技创新战略研究报告》，获教育部科技进步一等奖等省部级及其他奖项共4项。

1.    国家畜禽遗传资源委员会猪专业委员会委员

2.    全国科技平台标准化技术委员会委员

3.    第三次全国猪遗传资源普查技术专家组成员

4.    畜禽遗传改良北京市重点实验室副主任

5.    中国动物遗传育种学会理事

6.    全国动物生物技术学会理事

猪重要经济性状遗传基础解析，猪分子育种技术研究与新种质创制。

本科生课程：近十年课程数据
研究生课程：近十年课程数据

纵向项目 横向项目

论文题目	刊物名称	收录类别	发表年月	第一作者或全部作者	第一作者单位	排名

1. LuoY, HuangN, ZhaC, YangL, XueP, XuQ, YangX, ZhangL, WangY, ChaoZ, SunR, WangF, JiaS, FangM. Telomere-to-telomere genome assembly of a male pig provides insight into global population structure and selection for body stature. Nat Genet 58, 195–205 (2026).

2. Yang, X., Xie, Y., Luo, Y. et al. RFLNA mitigates heat stress-impaired chondrocyte proliferation and vertebral development through cytoskeletal regulation in pigs. J Animal Sci Biotechnol 17, 68 (2026).

3.  Xue, P., Zha, C., Luo, Y., Huang, N., Liu, N., Wang, H., & Fang, M. Genomics Insights into the Demographic History and Introgression of Tibetan Pigs. Animals : an open access journal from MDPI, 16(9), 1328  (2026).

4. Yang L, Cui R, Li Z, Xue M, Chan S, Xue P, et al. Gut Microbiota-Bile Acid Crosstalk Contributes to Meat Quality and Carcass Traits of Tan and Dorper Sheep. Int J Mol Sci. 2025 Jun;26(13):6224.

5. Du X, Liu Y, He X, Tao L, Fang M, Chu M. Identification and expression profile analysis of circRNAs associated with goat uterus with different fecundity during estrous cycle. BMC Genomics. 2025 Apr;26(1):349.

6. Yang, X., Luo, Y., Xue, M., Chan, S., Wang, Y., Yang, L., Zhang, L., Xie, Y., & Fang, M. (2024). Vitamin C Alleviates Heat-Stress-Induced Damages in Pig Thoracic Vertebral Chondrocytes via the Ubiquitin-Mediated Proteolysis Pathway. Antioxidants (Basel, Switzerland), 13(11), 1341.

7. Yang X, Yang S, Luo Y, et al. Single-cell transcriptional footprint for pseudogene SsCLEC9A is associated with antigen processing and presentation in Sus scrofa. Int J Biol Macromol. 2025;302:140629.

8. 郑美丽,王珏,雒亚彪,等. 基于全基因组SNPs遗传信息的猪品种保护策略研究 [J]. 中国畜牧杂志, 2025, 61 (01): 123-129. DOI:10.19556/j.0258-7033.20240527-06.

9. 杨丽贤,崔冉,薛明明,等. 多组学技术在绵羊肉质性状研究中的应用进展[J]. 中国畜牧杂志,2024,60(9):1-7.

10. Xie, F.; Wang, Y.; Chan, S.; Zheng, M.; Xue, M.; Yang, X.; Luo, Y.; Fang, M. Testosterone Inhibits Lipid Accumulation in Porcine Preadipocytes by Regulating ELOVL3. Animals 2024, 14, 2143.

11. Xue, P.; Xue, M.; Luo, Y.; Tang, Q.; Wang, F.; Sun, R.; Song, Y.; Chao, Z.; Fang, M. Colonic Microbiota Improves Fiber Digestion Ability and Enhances Absorption of Short-Chain Fatty Acids in Local Pigs of Hainan. Microorganisms 2024, 12, 1033.

12. Song Y, Xue M, Wang F, et al. Study on the Characteristics of Coarse Feeding Tolerance of Ding’an Pigs: Phenotypic and Candidate Genes Identification[J]. Genes, 2024, 15(5): 599.

13. Chan S, Wang Y, Luo Y, et al. Differential Regulation of Male-Hormones-Related Enhancers Revealed by Chromatin Accessibility and Transcriptional Profiles in Pig Liver[J]. Biomolecules, 2024, 14(4): 427.

14. Xue M, Huang N, Luo Y, et al. Combined Transcriptomics and Metabolomics Identify Regulatory Mechanisms of Porcine Vertebral Chondrocyte Development In Vitro[J]. International Journal of Molecular Sciences, 2024, 25(2): 1189.

15. He S, Wang Y, Luo Y, et al. Integrated analysis strategy of genome-wide functional gene mining reveals DKK2 gene underlying meat quality in Shaziling synthesized pigs[J]. BMC genomics, 2024, 25(1): 1-15.

16. 方美英,何帅涵,董柠其,等.新农科建设背景下《动物遗传学》思政教学的探索与实践[J].畜牧兽医杂志,2023,42(06):110-114+117.

17. 方美英,何帅涵,董柠其,等.新农科背景下动物科学专业课程思政教育的探索[J].养殖与饲料,2023,22(12):105-110.DOI:10.13300/j.cnki.cn42-1648/s.2023.12.016.

18. Li Z, Cui R, Wang Y B, et al. Specific gastrointestinal microbiota profiles in Chinese Tan sheep are associated with lauric acid content in muscle[J]. BMC microbiology, 2023, 23(1): 331.

19. Tang Q, Yu R, Wang Y, et al. Varied hypoxia adaptation patterns of embryonic brain at different development stages between Tibetan and Dwarf laying chickens[J]. BMC genomics, 2023, 24(1): 1-14.[1]

20. Xu Q, Luo Y, Chao Z, et al. Integrated Analysis of Tran scriptome Expression Profiles Reveals miRNA-326–NKX3.2-Regulated Porcine Chondrocyte Differentiation[J]. International Journal of Molecular Sciences, 2023,24(8):7257.

21. Luo Y, Xu Q, Xue M, et al. Novel Haplotype in the HHEX Gene Promoter Associated with Body Length in Pigs[J]. Genes, 2023,14(2):511.

22. Du X, Liu Y, He X, et al. Uterus proliferative period ceRNA network of Yunshang black goat reveals candidate genes on different kidding number trait[J]. Frontiers in Endocrinology, 2023,14:1165409.

23. Liu X, Bai Y, Cui R, et al. Sus_circPAPPA2 Regulates Fat Deposition in Castrated Pigs through the miR-2366/GK Pathway[J]. Biomolecules, 2022,12(6):753.

24. Xiao L, Xu Q, Liu X, et al. The Novel-miR-659/SPP1 Interaction Regulates Fat Deposition in Castrated Male Pigs[J]. Animals, 2022,12(8):944.

25. Liu X, Bai Y, Cui R, et al. Integrated Analysis of the ceRNA Network and M-7474 Function in Testosterone-Mediated Fat Deposition in Pigs[J]. Genes, 2022,13(4):668.

26. Cui R, Kang X, Liu Y, et al. Integrated analysis of the whole transcriptome of skeletal muscle reveals the ceRNA regulatory network related to the formation of muscle fibers in Tan sheep[J]. Frontiers in Genetics, 2022,13:991606.

27. 薛明明, 王克君, 产舒恒, 等. ARL15基因敲除小鼠模型建立和表型分析[J]. 农业生物技术学报, 2022,30(12):2376-2384.

28. 王余北, 肖莲梅, 何帅涵, 等. 基于50K SNP芯片信息的枣庄黑盖猪群体遗传结构分析[J]. 中国畜牧杂志, 2022,58(9):168-171, 178.

29. 方美英, 何帅涵, 汤启国, 等. 浅谈农科院校《动物遗传学》课程教学内容的思考与设计[J]. 教育现代化, 2022,9(13):129-132.

30. Tang Q, Xu Q, Ding C, et al. HIF-1 regulates energy metabolism of the Tibetan chicken brain during embryo development under hypoxia[J]. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2021,320(5):R704-R713.

31. Xu Q, Chen J, Liu X, et al. miR-F4-C12 Functions on the Regulation of Adipose Accumulation by Targeting PIK3R1 in Castrated Male Pigs[J]. Animals, 2021,11(11):3053.

32. Liu Y, Xu Q, Kang X, et al. Dynamic changes of genomic methylation profiles at different growth stages in Chinese Tan sheep[J]. Journal of Animal Science and Biotechnology, 2021,12(1):118.

33. Tang Q, Ding C, Xu Q, et al. Mitochondrial Fusion Potentially Regulates a Metabolic Change in Tibetan Chicken Embryonic Brain During Hypoxia[J]. Frontiers in Cell and Developmental Biology, 2021,9:585166.

34. Yang K, Wang J, Wang K, et al. Integrated Analysis of miRNA-mRNA Network Reveals Different Regulatory Patterns in the Endometrium of Meishan and Duroc Sows during Mid-Late Gestation[J]. Animals, 2020,10(3):420.

35. Wang J, Liu C, Chen J, et al. Genome-Wide Analysis Reveals Human-Mediated Introgression from Western Pigs to Indigenous Chinese Breeds[J]. Genes, 2020,11(3):275.

36. Yang W, Tang Q, Bai Y, et al. Bacterial magnetic particles-polyethylenimine vectors deliver target genes into multiple cell types with a high efficiency and low toxicity[J]. Applied Microbiology and Biotechnology, 2020,104(15):6799-6812.

37. Wang K, Yang K, Xu Q, et al. Protein expression profiles in Meishan and Duroc sows during mid-gestation reveal differences affecting uterine capacity, endometrial receptivity, and the maternal–fetal Interface[J]. BMC Genomics, 2019,20(1):991.

38. Xu Q, Liu X, Chao Z, et al. Transcriptomic Analysis of Coding Genes and Non-Coding RNAs Reveals Complex Regulatory Networks Underlying the Black Back and White Belly Coat Phenotype in Chinese Wuzhishan Pigs[J]. Genes, 2019,10(3):201.

39. 王珏, 刘成琨, 刘德武, 等. 基于不同密度SNP芯片在杜洛克公猪中的全基因组选择效果分析[J]. 中国畜牧杂志, 2019,55(12):75-79.

40. 徐芹芹, 刘玉芳, 康晓龙, 等. 滩羊EphA3基因克隆表达分析及生物信息学初步研究[J]. 中国畜牧杂志, 2019,55(3):33-38.

41. 王继英, 成建国, 林松, 等. 枣庄黑盖猪亲缘关系分析及分子系谱构建[J]. 猪业科学, 2019,36(8):136-138.

42. Liu Y, Zhang J, Xu Q, et al. Integrated miRNA-mRNA analysis reveals regulatory pathways underlying the curly fleece trait in Chinese tan sheep[J]. BMC Genomics, 2018,19(1):360.

43. Meng Q, Wang K, Liu X, et al. Identification of growth trait related genes in a Yorkshire purebred pig population by genome wide association studies[J]. Asian-Australasian Journal of Animal Sciences, 2017,30(4):462-469.

44. Wang K, Liu Y, Xu Q, et al. A post-GWAS confirmingGPAT3gene associated with pig growth and a significant SNP influencing its promoter activity[J]. Animal Genetics, 2017,48(4):478-482.

45. Kang X, Liu Y, Zhang J, et al. Characteristics and Expression Profile of KRT71 Screened by Suppression Subtractive Hybridization cDNA Library in Curly Fleece Chinese Tan Sheep[J]. DNA and Cell Biology, 2017,36(7):552-564.

46. Wang K, Li W, Bai Y, et al. ssc-miR-7134-3p regulates fat accumulation in castrated male pigs by targeting MARK4 gene[J]. International Journal of Biological Sciences, 2017,13(2):189-197.

47. Liu Y, Kang X, Yang W, et al. Differential expression of KRT83 regulated by the transcript factor CAP1 in Chinese Tan sheep[J]. Gene, 2017,614:15-20.

48. Hayward J J, Castelhano M G, Oliveira K C, et al. Complex disease and phenotype mapping in the domestic dog[J]. Nature Communications, 2016,7(1):10460.

49. Yang W, Bai Y, Wang X, et al. Attaching Biosynthesized Bacterial Magnetic Particles to Polyethylenimine Enhances Gene Delivery Into Mammalian Cells[J]. Journal of Biomedical Nanotechnology, 2016,12(4):789-799.

50. 方美英, 邓学梅, 吴克亮, 等. 动物遗传学课程课堂讨论的组织与实施[J]. 高等农业教育, 2016(6):67-69.

51. 方美英, 刘剑锋, 张勤, 等. 加速优秀基因传递 开辟动物遗传育种新时代[J]. 中国农村科技, 2016(6).

52. Wang K, Liu D, Hernandez-Sanchez J, et al. Genome Wide Association Analysis Reveals New Production Trait Genes in a Male Duroc Population[J]. PLOS ONE, 2015,10(9):e139207.

53. Chen G, Bai Y, Ren L, et al. Metabolism of Androstenone, 17β-Estradiol and Dihydrotestosterone in Primary Cultured Pig Hepatocytes and the Role of 3β-Hydroxysteroid Dehydrogenase in This Process[J]. PLoS ONE, 2015,10(1):e113194.

54. Bai Y, Huang J, Liu G, et al. A comprehensive microRNA expression profile of the backfat tissue from castrated and intact full-sib pair male pigs[J]. BMC Genomics, 2014,15(1):47.

55. Kang X, Liu G, Liu Y, et al. Transcriptome Profile at Different Physiological Stages Reveals Potential Mode for Curly Fleece in Chinese Tan Sheep[J]. PLoS ONE, 2013,8(8):e71763.

56. Yang W, Kang X, Yang Q, et al. Review on the development of genotyping methods for assessing farm animal diversity[J]. Journal of Animal Science and Biotechnology, 2013,4(1):2.

57. 陈洁, 白莹, 徐芹芹, 等. 猪PLSCR4基因外显子区PCR-RFLP遗传多态性分析[J]. 中国畜牧杂志, 2013,49(11):1-4.

58. 方美英. 中国地方猪种对世界猪种培育的贡献-来自分子遗传及进化数据的分析[J]. 中国猪业, 2013(S1):16-19.

59. Chen G, Li S, Dong X, et al. Investigation of Testosterone, Androstenone, and Estradiol Metabolism in HepG2 Cells and Primary Culture Pig Hepatocytes and Their Effects on 17βHSD7 Gene Expression[J]. PLoS ONE, 2012,7(12):e52255.

60. Dong X, Bai Y, Xin Y, et al. Investigation on the transcription factors of porcine 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase genes[J]. Gene, 2012,499(1):186-190.

61. Huang J, Yang Y, Liu G, et al. Molecular cloning and characterization of the porcine FTO promoter and coding regions[J]. Molecular Biology Reports, 2011,38(4):2855-2862.

62. 董新星, 方美英, 陈刚. 3β和17β羟基类固醇脱氢酶的研究进展[J]. 中国畜牧兽医, 2011,38(8):66-70.

63. 杨云周, 张吉斌, 方美英. 低氧诱导因子-1α基因在藏鸡低氧适应机制中的研究进展[J]. 中国畜牧兽医, 2011(12):84-87.

64. Huang J, Liu G, Liu Y, et al. Splice Variant Identification and Expression Analysis of the Fat Mass and Obesity-Associated (FTO) Gene in Intact and Castrated Male Pigs[J]. DNA and Cell Biology, 2010,29(12):729-733.

65. 张吉斌, 刘月平, 方美英. 线粒体ATP合酶基因组成及生化机制研究进展[J]. 中国畜牧杂志, 2010,46(7):64-68.

66. Fang M, Larson G, Ribeiro HS, Li N, Andersson L. Contrasting mode of evolution at a coat color locus in wild and domestic pigs. PLoS Genet. 2009 Jan;5(1):e1000341.

67. Cho I, Han S, Fang M, et al. The robust phylogeny of Korean wild boar (Sus scrofa coreanus) using partial D-loop sequence of mtDNA[J]. Molecules and Cells, 2009,28(5):423-430.

68. 樊宝良, 张磊, 方美英, 等. 中国牛科主要饲养品种朊蛋白基因种系发生分析[J]. 农业生物技术学报, 2009,17(2):363-364.

69. Fang M, Andersson L. Mitochondrial diversity in European and Chinese pigs is consistent with population expansions that occurred prior to domestication[J]. Proceedings of the Royal Society B: Biological Sciences, 2006,273(1595):1803-1810.

70. THULIN C, FANG M, O. AVERIANOV A. Introgression from Lepus europaeus to L. timidus in Russia revealed by mitochondrial single nucleotide polymorphisms and nuclear microsatellites[J]. Hereditas, 2006,143(2006):68-76.

71. G. Larson, K. Dobney, U. Albarella, M. Fang, E. Matisoo-Smith, J. Robins, S. Lowden, H. Finlayson, T. Brand, E. Willerslev, P. Rowley-Conwy, L. Andersson and A. Cooper, Worldwide phylogeography of wild boar reveals multiple centers of pig domestication[J].Science 307 (2005) 1618–21.

72. Y M, Braunschweig M, Hu X, et al. Genetic Variation of Exon 2 of SLA-DQB Gene In Chinese Pigs[J]. Biochemical Genetics, 2005,43(3-4):119-125.

73. 强巴央宗, 凌遥, 方美英. 藏猪全同胞和半同胞亲缘关系鉴定[J]. 中国畜牧杂志, 2003,39(4):31, 33.

74. Fang M Y, Hu X X, Li N, et al. [Polymorphism analysis of the exon 2 of SLA-DQB gene in Xiao Meishan, Zhong Meishan and Yorkshire pigs with PCR-RFLP][J]. Yi Chuan Xue Bao, 2002,29(8):685-687.

75. 方美英, 赵兴波, 李宁, 等. 不同品种猪解耦联蛋白基因3′调控区的遗传变异[J]. 科学通报, 2002,47(13):1010-1012.

76. 方美英, 胡晓湘, 李宁, 等. 小梅山、中梅山及大约克猪的SLA-DQB基因外显子2 PCR-RFLP多态性分析[J]. 遗传学报, 2002,29(08):685-687.

77. 方美英, 吴常信. 猪的毛色遗传[J]. 中国畜牧杂志, 2002,38(2):51-52.

78. 方美英, 吴常信. 猪品种遗传多样性的研究进展[J]. 畜牧与兽医, 2001,33(5):40-42.

79. 方美英, 姜志华, 刘红林, 等. 不同猪种中氟烷基因频率调查分析[J]. 浙江农业学报, 1999,11(3):145.

80. 方美英, 刘红林, 姜志华, 等. 6个猪种胰岛素样生长因子-1(IGF-1)基因座位遗传多态性检测[J]. 畜牧与兽医, 1999,31(01):14-15.

81. 陈杰, 姜志华, 刘红林, 等. 二花脸猪FSH β亚基位点PCR-SSCP标记与产仔数关系初探[J]. 南京农业大学学报, 1999,22(2):55.

82. 姜勋平, 张牧, 刘桂琼, 等. 猪RYR1基因对繁殖性能的影响[J]. 江苏农业研究, 1999,20(1):48-51.

83. 方美英, 姜志华. 六个品种猪生长激素基因座位遗传多态性检测[J]. 动物学研究, 1998,19(4):334-336.

84. 曹建国, 赵芳根, 邵雪忠, 等. 利用猪毛PCR-RFLPs方法检测氟烷基因[J]. 上海农业学报, 1998(04):23-26.

85. 方美英, 姜志华, 刘红林, 等. 应用PCR-SSCPS、PCR-RFLPS技术检测猪氟烷基因[J]. 养猪, 1997(1):28-29.

86. 朱婉茹, 姜志华, 刘红林, 等. 金华猪、皖浙花猪和长白猪氟烷基因检测(简报)[J]. 农业生物技术学报, 1996(03):299-300.

87. 董新星, 辛毅, 白莹, 等. 3HSDs1和17HSDs7基因5'上游区转录活性研究[C], 北京, 2010.2010.

88. 董新星, 白莹, 杨云周, 等. 猪3βHSD1基因转录因子鉴定分析[C], 扬州, 2011.

89. 杨云周, 白莹, 董新星, 等. 藏鸡HIF-1α和HIF-2α基因在低氧中的表达研究[C], 扬州, 2011.

90. 董新星, 刘刚, 白莹, 等. 利用焦磷酸测序技术分析猪IGF-2基因3072位点的遗传变异[C], 南京, 2010.

黄金明, 刘刚, 刘月平, 等. 猪肥胖相关基因(FTO)剪切体鉴定及其在阉割与非阉割公猪中的表达分析: 第十五次全国动物遗传育种学术讨论会[C], 中国陕西杨凌, 2009.

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